This page is a room-by-room breakdown of Underground Sorcery Lab. All "recurring features" of a given room — namely, enemies that respawn whenever the room is reentered and blue chests that self-refill whenever the zone is exited and reentered — are listed in bold above one-time collectibles and events. All chest icons shown in the map screen below are the zone's blue chests.
- Screen 1:
- ×2 Bomber Morte, ×2 Sidhe, ×1 Puppy
- ×2 Bomber Morte, ×1 Cyhyraeth (Hard)
- Blue chest containing Black Pepper, Curry Powder, Walnut, and/or Damascus
- Exit to Bridge of Evil
- Screen 2:
- ×6 Gusion
- Cutscene with Dominique Baldwin
- Screen 3:
- ×4 Poison Toad, ×1 Bomber Morte
- ×2 Cyhyraeth (Hard)
- Blue chest containing Black Pepper, Curry Powder, Walnut, and/or Damascus
- Bookcase: The journal of an unnamed alchemist Vol. 5
- Exit to Screen 7 at the upper right (requires Reflector Ray/Double Jump)
- Screen 4:
- Bathin (boss), dropping Reflector Ray shard
- Screen 5:
- ×4 Sidhe, ×2 Bomber Morte, ×1 Puppy, ×1 Mimic
- ×1 Shield Outsider (Hard)
- HP Max Up
- Breakable floor leading to Screen 6
- Green chest containing Silver Power Ring (requires Reflector Ray)
- Bookcase about Force Blast technique
- Screen 6:
- Green chest containing Skull Necklace
- MP Max Up
- Screen 7:
- ×1 Macaron
- Green chest containing Damascus Equipment/R
- Breakable wall leading to Screen 8
- Screen 8: